Year: 2018 | Month: August | Volume 8 | Issue 4
Developmental Potential and Apoptosis Incidence of In Vitro Produced Buffalo Embryos Vitrified by Solid Surface Technique
Abstract:
Under the present study the post-thawed developmental potential and apoptotic incidence of in vitro-produced (IVP) buffalo embryos vitrified by solid surface technique were assessed. The abattoir derived oocytes were in vitro matured in maturation media and fertilized with capacitated epididymal sperm. The fertilized embryos were cultured in commercial Research vitro cleave media for in vitro embryo development. Embryos at morula stages were vitrified using vitrification media composed of 35% Ethylene glycol. The vitrified morulae were thawed and allowed to grow in culture media similar to non-vitrified control embryos up to the blastocyst stage. The post-thawed developmental competence of vitrified embryos was recorded to be significantly lower in terms of compact morula (57±1.22% vs 81±1.87%), blastocyst (32±1.22% vs 58±1.22%) and blastomere count (129.0±1.22 vs 159.2±1.31) as compared to non-vitrified control. Furthermore, Annexin-V and PI staining dye were used for staining the embryos for apoptotic study under confocal microscope. The apoptotic study of embryos observed by confocal microscopy revealed that rate of apoptosis was significantly higher in vitrified group as compared to control (53.33±2.13% vs 26.67±3.43%). Our study concluded that the vitrification increased the rate of apoptosis which significantly affected the development potential of vitrified-thawed embryos.
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